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   Today: Last Update: 01 April 2013
 
 
   
 

Exemplary Results obtained in-house

 

(1) Knock-down efficiency

two shRNAmirs targeting human SNX17
courtesy of Dr. Andrea Bugarcic, IMB

RNAi efficiency of two shRNAmir targeting human SNX17. Two colour Western Blot using the Odyssey system.

shRNAmir targeting human Vps26A, Vps29, Vps35
courtesy of Dr. Andrea Bugarcic, IMB



Two colour Western Blot using the Odyssey system. Vps26A, Vps29, and Vps35 are present within the cell as a protein complex; RNAi successfully targeting either one also reduces protein levels of the other ones. Lentivirus targeting human Vps26A, Vps29, and Vps35 is available from LISA as small aliquot positive controls.

shRNAmirs "B2" and "B4" in 3 different cell lines, no selection, functional assay
courtesy of Stephen Ainger, IMB

Melanocytes B2B4

 

(2) Transduction Efficiency

 

 

(3) Stable cell lines

MM96L
courtesy of Stephen Ainger, IMB

Melanocytes Stable 001